Semi-Micro vs. Conventional TSK-GEL Columns
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Comparison of Semi-micro Columns TSK-GEL vs. Conventional TSK-GEL GPC columns
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Conventional
TSK-GEL Columns |
TSK-GEL
SuperMultiporeHZ-N |
| Particle size |
5 µm |
3 µm |
| Theor. plates (N/column) |
16,000 |
16,000 |
| Dimensions |
7.8 mm ID x 30 cm L |
4.6 mm ID x 15 cm L |
| Flow rate |
1.0 mL/min |
0.35 mL/min |
| Solvent consumption* |
11.5 mL/analysis |
2.0 mL/analysis |
| Injection volume |
50 µL/column |
50 µL/column |
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*Based on a mobile phase porosity (εt) of 0.8, and the listed column dimensions.
Comparison of run times: TSK-GEL Semi-micro vs. Conventional columns
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Column: |
(A) TSKgel SuperMultiporeHZ-M (4.6 mm ID x 15 cm L x 2)
(B) TSKgel SuperMultiporeHZ-M (4.6 mm ID x 15 cm L x 4)
(C) TSKgel SuperMultiporeHXL-M (7.8 mm ID x 30 cm L x 2) |
| Eluent: |
THF |
| Flow rate: |
(A) 0.35 mL/min
(B) 0.35 mL/min
(C) 1.00 mL/min |
| Temp.: |
40°C |
| Detection: |
RI (HLC-8120GPC) |
| Sample: |
PTMEG* 650 (1.0%) |
| Inj.Vol.: |
(A) & (B) 10 µL (C) 35 µL |
| * poly (tetramethylene ether glycol) |
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GPC Analysis of PTMEG
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Column: |
TSKgel SuperMultiporeHZ-N |
| Eluent: |
THF |
| Flow rate: |
A: 0.35 ml/min
B: 1 ml/min |
| Detection: |
RI |
| Temperature: |
40°C |
| Inj. volume: |
A: 10 µl; B: 60 µl |
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