Semi-Micro vs. Conventional TSKgel Columns
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Benefits of semi-micro columns
The columns of choice for use with the EcoSEC GPC system are referred to as semi-micro columns (dimensions are smaller than conventional columns in terms of internal diameter as well as length: 4.6 mm or 6 mm ID x 15 cm L).
Semi-micro column dimensions are advantageous in terms of shorter analysis time (more samples analyzed per unit time), lower solvent consumption and reduced solvent disposal cost. The EcoSEC GPC system is designed to achieve these benefits. Other GPC systems must be optimized with respect to delay or dead volume as this results in extra-column peak broadening, thus lowering resolution, accuracy and precision.
Minimal extra-column band broadening is required to take full advantage of the highest efficiency GPC columns available. The EcoSEC GPC system uses state of the art fluidics and an innovative design that results in low dead volume. Together with a small stroke volume pump and a 2.5 µL RI flow cell, the EcoSEC GPC system delivers short analysis times, excellent reliability and low operating cost. Semi-micro TSK-GEL GPC columns, 4.6 mm ID x 15 cm L, are at the heart of the EcoSEC GPC system.
Effect of system dead volume
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Column: |
TSKgel SuperHZ2000 (4.6 mm ID x 15 cm L x 4) |
| Eluent: |
THF |
| Flow rate: |
0.35 mL/min |
| Temp.: |
40°C |
| Detection: |
RI |
| Sample: |
polystyrene oligomer (MW-500) |
| Inj.Vol.: |
10 µL (0.2 g/L) |
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TSKgel columns for polymers soluble in polar organic solvents
Semi-micro columns (6 mm ID x 15 cm L)
TSKgel SuperAW line
Conventional columns (7.5 mm ID x 30 cm L)
TSKgel Alpha columns
TSKgel columns for polymers soluble in organic solvents
Semi-micro columns (4.6 or 6 mm ID x 15 cm L)
TSKgel SuperMultiporeHZ line
TSKgel SuperHZ line for ultra-low adsorption characteristics
TSKgel SuperH line for low adsorption characteristics; max. temp. 140ºC
Conventional columns (7.8 mm ID x 30 cm L)
TSKgel Hxl columns for ultra-low adsorption characteristics
TSKgel Hhr columns for low adsorption characteristics; max. temp. 140ºC
Our flagship TSKgel SW-type columns for the analysis of proteins, and the TSKgel PW-type columns for analyzing water-soluble polymers can be found here.
To find out which TSKgel column best suits your SEC analysis, visit the Tosoh Bioscience Website.
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